Fluorescent In Situ Hybridization (FISH) Fluorescent in situ hybridization (FISH) is a fluorescence probe
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چکیده
The importance of cancer biomarkers is now increasingly recognized and acted on by regulatory organizations, healthcare providers (from reimbursement through to practicing clinicians), cancer organizations, research institutes, and charities. For example, the American Joint Committee on Cancer (AJCC) states in the opening of its mission statement, “The purpose and scope of the AJCC is to create and promote a taxonomy of cancer patient groups that accurately predicts outcomes by incorporating traditional (TNM) and newer data in a timely and dynamic process.” Here, newer data embrace the burgeoning cancer biomarker field.
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دورگهسازی در محل؛ اصول و کاربردها : مقاله مروری
In situ hybridization (ISH) is a method that uses labeled complementary single strand DNA or RNA to localize specific DNA or RNA sequences in an intact cell or in a fixed tissue section. The main steps of ISH consist of: probe selection, tissue or sample preparation, pre-hybridization treatment, hybridization and washing, detection and control procedure. Probe selection is one of the important ...
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